Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 6;7:306. doi: 10.3389/fcimb.2017.00306

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Rodriguez-Rodrigues, Castillo, Landoni, Martire-Greco, Milillo, Barrionuevo and Fernández.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

pRNA-induced ROS requires priming. (A) Representative dot-plot showing the SSC vs. FSC profile of the different populations observed in whole blood assays after lysis of erythrocytes using a commercial lysing buffer. The PMN population is indicated. (B) Whole blood was incubated with pRNA and/or the priming stimulus, TNF-α, for 30 min and the expression of CD11b was measured by flow cytometry within the gate of PMN. A histogram of one representative experiment out of three is shown. (C) Whole blood was incubated with DHR for 15 min and with the different stimuli for additional 30 min. The % of DHR+ PMN was determined by flow cytometry within the gate of PMN. Dot-plots of the percentage of DHR+ PMN of one representative experiment out of three are shown. In all cases ^*p < 0.05 vs. all other groups.