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. 2017 Jul 5;7:4663. doi: 10.1038/s41598-017-04946-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Change in body weight in sham-operated mice and ovariectomized mice administered placebo, C-1, or E2. Mice administered C-1 or E2 had significantly lower body weight at day 14 than those administered placebo. Error bars represent standard errors of the mean. Post-hoc LSD test: *P < 0.05 as compared with sham-operated mice; ***P < 0.001 as compared with sham-operated mice; ****P < 0.0001 as compared with sham-operated mice; ^# P < 0.05 as compared with placebo-administered mice; ^### P < 0.001 as compared with placebo-administered mice.