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. 2017 Jul 5;7:4663. doi: 10.1038/s41598-017-04946-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Results of forced swim test during 0–3 minutes in sham-operated mice and ovariectomized mice administered placebo, C-1, or E2. The bar graph shows the mean immobility time during 0–3 minutes of the forced swim test for control mice and ovariectomized mice administered placebo, C-1, or E2. The immobility time was significantly shorter in mice administered C-1 or E2 than in those administered placebo. Error bars represent standard errors of the mean. Post-hoc LSD test: **P < 0.01 as compared with sham-operated mice; ^# P < 0.05 as compared with placebo-administered mice; ^## P < 0.01 as compared with placebo-administered mice.