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. 2017 Jul 5;7:4712. doi: 10.1038/s41598-017-04967-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characteristics of CDS cells. (a) Phase-contrast images of CDS cells cultured in conventional culture conditions and (b) in the ultra-low attachment culture dishes. Bar: 500 μm. (c,d) The growth curves for NCC-CDS1-X1-C1 (c) and X3-C1 (d) cells. (e,f) Immunohistochemical analysis for WT1 (e) and ETV4 (f). (g) CIC break-apart FISH of CDS cells. The arrows indicate the splits. (h) Sanger sequence analysis of the transcript showing CIC–DUX4 fusion (upper, NCC-CDS1-X1-C1; lower, NCC-CDS1-X3-C1). An antiparallel 4767–4774 region of CIC exon insertion is indicated in the orange box.