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. 2017 Jul 5;7:4739. doi: 10.1038/s41598-017-04972-3

Figure 2.

Figure 2

Silencing Vgat-Cre + SDH interneurons elicits spontaneous nocifensive behaviours. (a,b) Spontaneous nocifensive behaviours, licking and biting (a) and flinching (b) after i.p. injection of saline or CNO (10 mg kg−1) in Vgat-Cre;AAV-hM4DiFLEX mice. These behaviours were assessed for 1 hr after CNO injection (n = 4–6; *P < 0.05, **P < 0.01, ***P < 0.001 vs. saline). (c) c-Fos immunoreactivity in the SDH of Vgat-Cre;AAV-hM4DiFLEX mice 90 min after administration of saline or CNO (10 mg kg−1, i.p.). (d) Quantification of c-Fos+ cells in the SDH (n = 5, ***P < 0.001). (e) Effects of intrathecal pretreatment with muscimol, a GABAA receptor agonist, on the CNO-induced nocifensive behaviours (left, licking and biting; right, flinching); n = 5; *P < 0.05, **P < 0.01, ***P < 0.001, # P < 0.05 vs. CNO + PBS. (f,g) Effect of intrathecal pretreatment with muscimol (1 and 2 μg) on the CNO-induced increase in c-Fos+ cells. c-Fos immunoreactivity in the SDH (f) and quantification ((g) n = 5, *P < 0.05). Data are means ± SEM.