Figure 6.

Effect of N-glycan deletions on ligand-induced conformational change of α_IIbβ₃ integrin. (A–D) The binding of the active conformation-specific anti-β₃ mAb 319.4 (A,C) and the anti-α_IIb mAb 370.3 (B,D) to the indicated α_IIbβ₃ constructs expressed in HEK293FT cells in the absence or presence of ligand-mimetic drug eptifibatide (Ept) plus 1 mM Ca²⁺/Mg²⁺. The mAb binding was measured by flow cytometry and presented as a normalized MFI to integrin expression (AP3 binding). Data are means ± s.e.m. (n = 3). Two-tailed t-tests were used to compare the wild type (WT) and the mutants in the same condition. *P < 0.05.