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. 2017 Jul 6;11:388. doi: 10.3389/fnins.2017.00388

Figure 2.

Figure 2

Neuronal death and astrogliosis following prolonged OGD. (A) Cartoon of an experimental modeling of prolonged ischemic conditions (30-min duration) in organotypic hippocampal slice cultures for long-term assessment of ischemic CA1 neuronal death. (B) Immunohistology with the neuronal marker, NeuN (left images), and its quantitative analysis in CA1 area in control (n = 7 slices) and after prolonged OGD (n = 3 slices exposed to 30-min OGD; right graphs). Scale bar, 100 μm. Data are mean with s.e.m. ***P < 0.001 (t-test). (C) Immunohistology with astrocytic marker GFAP in control organotypic slice and one exposed to prolonged OGD 1 week-post. Note a prominent outgrowth of GFAP-positive cells after OGD. Scale bar, 50 μm; insert, 20 μm.