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. 2017 Jul 6;11:388. doi: 10.3389/fnins.2017.00388

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Copyright © 2017 Rybachuk, Kopach, Krotov, Voitenko and Pivneva.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mild OGD triggers the delayed CA1 neuronal death. (A) Immunohistology with the neuronal marker, NeuN (left images), and quantitative analysis of the NeuN-positive cells in CA1 area in control (n = 7 slices) and after mild OGD (n = 7 slices exposed to 10-min OGD; right graphs). Scale bar, 20 μm. Data are mean with s.e.m. (B) Quantitative analysis of the caspase-3-positive cells in control (n = 4) and 1–2 weeks post-OGD (n = 10 slices exposed to 10-min OGD). The numbers of fluorescent-labeled cells are normalized to the correspondent value at 1 week post-OGD. (C) Immunohistology with the astrocytic marker, GFAP, and the microglial marker, Iba-1, before and 2 weeks after mild OGD. Scale bar, 50 μm. ^*P < 0.05, ^**p < 0.01, ^***p < 0.001 (unpaired t-test compared with corresponding control as indicated).