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. 2017 Jul 5;7:4718. doi: 10.1038/s41598-017-05088-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Additional deletions observed in LdCen ^−/− mutant in Chromosomes 16. (A) The deletions predicted by the bioinformatics analysis are shown as red bars. The gene arrangement in the reference L. donovani genome with the gene listing is shown as a black bar. The open reading frames are shown as green bars and the untranslated regions (UTR) are shown as blue bars. The size of the deletion is also indicated. The predicted hypothetical protein coding genes are indicated with the gene identifiers are shown. (B) The restriction map upon SacI digestion is shown. The location of the probe used in hybridization experiments is indicated with red. The predicted size of the fragment upon Southern hybridization is indicated as black arrows. (C) Southern hybridization of SacI digested L. infantum, LdWT and LdCen ^−/− gDNA is shown. Absence of the 4.0 kb and 2.3 kb bands is indicated with a red arrow. Additional deletions in LdCen ^−/− genome ranging from 700–900 bp found in chromosomes (D) 33 and (E) 8 and the nearest predicted protein coding ORFs are indicated.