Skip to main content
. 2017 Jul 5;7:4653. doi: 10.1038/s41598-017-04748-9

Figure 3.

Figure 3

Dose response study of various molecules by employing EMSA. (a) Compound I-20, (b) Compound I-42. Dotted lines represent two different polyacrylamide gels. Lane 1-Free DNA, Lane 2-Protein+DNA, Lane 3-Protein+DNA+DMSO (−ve control), Lane 4–100 µg/ml, Lane 5–50 µg/ml, Lane 6–25 µg/ml, Lane 7–12.5 µg/ml, Lane 8–6.25 µg/ml, Lane 9–3.12 µg/ml, Lane 10–1.56 µg/ml, Lane 11–0.78 µg/ml, Lane 12–0.39 µg/ml (only in Fig. 3b). (For IC50 calculations, Protein+DNA and Protein+DNA+DMSO, control reactions were loaded in every gel and percentage of bound DNA for each inhibitor concentration was calculated by comparing the control reactions loaded in the same gel).