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. 2017 Jul 5;7:4653. doi: 10.1038/s41598-017-04748-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of inhibitory potential of compound I-108 against the growth of intracellular M. tuberculosis. (a) The influence of varying concentrations of I-108 on the growth of intracellular M. tuberculosis as analyzed on the agar plates. Panel a depicts the emergence of pin point colonies at the concentrations of 150 μg/ml and 125 μg/ml as compared to prominent growth of bacilli at lower concentrations of I-108. (b) Alamar blue assay to measure the viability of uninfected THP-1 cells in the presence of varying concentrations of I-108. The upper lane of panel b depicts the various concentrations of I-108 employed for the alamar blue assay (in μg/ml). Lanes 1–6 in panel a depicts following concentrations of I-108: Lane 1–150 μg/ml, Lane 2–125 μg/ml, Lane 3–100 μg/ml, Lane 4–50 μg/ml, Lane 5–25 μg/ml, Lane 6- -ve (only cells).