Table 3.
Sequencing data profiles and alpha diversity indexes.
US | SKa | DT | SS | SE | AH | |
---|---|---|---|---|---|---|
Number of raw sequences | 1,053,625 | 480,983 | 1,028,376 | 1,761,134 | 1,167,238 | 1,100,225 |
Average size read (bp) | 35–301 | 35–301 | 35–301 | 45–301 | 35–301 | 35–301 |
Mean GC content (%) | 58 | 56 | 54 | 53 | 54 | 55 |
High quality sequencesb | 1,013,171 | 429,677 | 952,470 | 1,650,101 | 1,084,632 | 1,057,048 |
Cleaned sequencesc | 990,519 | 424,188 | 914,072 | 1,627,045 | 987,849 | 984,566 |
PyNAST aligned sequences | 987,974 | 421,383 | 912,827 | 1,608,109 | 985,204 | 982,466 |
Sequences without singletons | 974,062 | 417,783 | 898,358 | 1,569,331 | 970,574 | 968,368 |
Total OTUsd | 7,326 | 6,334 | 9,083 | 26,244 | 11,504 | 6,430 |
Taxonomy assigned sequences | 801,105 | 376,334 | 861,537 | 1,264,280 | 912,975 | 909,979 |
Bacteria | 785,038 | 373,507 | 857,088 | 1,243,916 | 908,541 | 909,890 |
Archaea | 16,067 | 2,827 | 4,449 | 20,364 | 4,434 | 89 |
Unassigned sequences | 172,957 | 41,449 | 36,821 | 305,051 | 57,599 | 58,389 |
ALPHA DIVERSITY ANALYSIS | ||||||
Good's coverage | 0.985 | 0.987 | 0.983 | 0.974 | 0.982 | 0.985 |
Shannon-Wiener | 6.505 | 9.020 | 6.123 | 9.929 | 7.128 | 7.513 |
Simpson | 0.962 | 0.992 | 0.933 | 0.984 | 0.955 | 0.977 |
Data collected from Bioproject accession number PRJEB7059.
Sequences which passed the quality filtration and sequence read merging process.
Sequences obtained after chimera removal.
Amount of observed OTUs after singletons removal.