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. 2017 Jul 5;7:4693. doi: 10.1038/s41598-017-04944-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR validation of differentially expressed genes. (A) qRT-PCR validation of unigenes associated with resistance to PWN. (B) Correlation of 43 unigene expression results obtained from qPCR and RNA-seq. Relative expression levels of qRT-PCR calculated using Elongation factor 1-alpha as the internal control. The data are expressed as the mean (±SE) of three replicates. The expression data are presented as expression values of genes in the stigma sample relative to their expression. Error bars represent the SE.