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. 2017 Jul 5;7:4685. doi: 10.1038/s41598-017-04002-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of SQD2.2 resulted in a reduced seed setting rate and tiller number. (A) Identification of transgenic plants using PCR. (B) and (C) SQD2.2 overexpression in the transgenic plants was confirmed via semi-quantitative RT-PCR and quantitative real-time PCR. Values are the mean ± standard error (SE) (n = 3). (D) and (E) Seed setting rate. Values are the mean ± SE (n = 10). (F) Seed yield per plant. Values are the mean ± SE (n = 10). (G) The tiller number per plant. Values are the mean ± SE (n = 6). (H) Shoot dry weight per plant. Values are the mean ± SE (n = 10). Student’s t test; **P < 0.01. Scale bar = 5 cm.