Table 3.

Linearity data of chromatographic UPLC-ESI-MS/MS and HPTLC Method for mixed standards (n = 3).

Linearity parameters	Solasonine		Solamargine		Solasodine
	UPLC-ESI-MS/MS (ng/mL)	HPTLC (ng/spot)	UPLC-ESI-MS/MS (ng/mL)	HPTLC (ng/spot)	UPLC-ESI-MS/MS (ng/mL)	HPTLC (ng/spot)
Linearity	5–100	16.6–1,328	5–100	16.6–1,328	5–100	16.6–1,328
Equation	Y = 142.53x + 751.37	Y = 179.400 + 1.955x	Y = 348.83x + 4,834.9	Y = 263.823 + 2.080x	Y = 3,477.8x + 32,841	Y = 547.683 + 4.225x
Regression ± SD	0.990 ± 0.001	0.997 ± 0.001	0.992 ± 0.0005	0.997 ± 0.0005	0.994 ± 0.0001	0.993 ± 0.0001
Slope ± SD	142.53 ± 0.002	179.400 ± 0.002	348.83 ± 0.003	263.823 ± 0.003	3,477.8 ± 0.001	547.683 ± 0.001
Intercept ± SD	751.37 ± 0.002	1.955 ± 0.002	4,834.9 ± 0.005	2.080 ± 0.005	32,841 ± 0.001	4.225 ± 0.001
LOD	0.37	2.48	0.33	3.33	0.02	3.89
LOQ	1.15	7.52	1.01	10.09	0.06	11.18

LOD, limit of detection; LOQ, Limit of quantification; SD, standard deviation; RSD, Relative.