Table 1.
Common MALDI matrices used in glycomics and glycoproteomics
| Chemical name | Name | Solventa | Notes |
|---|---|---|---|
| 2,5-Dihydroxybenzoic acid | DHB | 50:50 water/acetonitrile | Glycans, glycopeptides [65] |
| 2,4,6-Trihydroxyacetophenon | THAP | 50:50 water/acetonitrile with 0.1% TFA | Glycoproteins, glycopeptides [66] |
| α-Cyano-4-hydroxycinnamic acid | CHCA | 50:50 water/acetonitrile with 0.1% TFA | Glycopeptides [65] |
| 3,5-Dimethoxy-4-hydroxycinnamic acid | Sinapinic acid | 70:30 water/acetonitrile with 0.1% TFA | Large proteins [67] |
| 3-Aminoquinoline/α-cyano-4-hydroxycinnamic acid | 3-AQ/CHCA | 9:9:2 water/acetonitrile with NH4H2PO4 | Glycans [68] |
| 2,5-Dihydroxybenzoic acid/N,N-dimethylaniline | DHB/DMA (3:1) | 1:1 water/acetonitrile | Glycans [69] |
aDissolve 10–20 mg in 1 mL of solvent; some matrices may not dissolve completely