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. 2017 Jul 5;7:4716. doi: 10.1038/s41598-017-05096-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nopal consumption decreases lipogenesis, hepatic steatosis and inflammation. Relative expression of (A) Srebp-1, (B) Acetyl-CoA carboxylase Acc, (C) Fas, (D) Ppar-α and (E) Cpt-1 in liver of Wistar rats fed one of the following diets: control diet (C), high fat diet with 5% sucrose in drinking water (HFS), HFS + 5% nopal (HFS + N), and obese rats switched to C diet with (HFS-C + N) or without (HFS-C) 5% nopal. (F–J) Hematoxilin-Eosin staining (HE) and (K–O) immunohistochemistry of TNF-α in liver of these rats. Data are expressed as mean ± SEM (n = 5/group). Comparisons among groups were analysed by 2-way ANOVA followed by Fisher’s PLSD test. Different letters indicate significant differences among groups (a > b > c > d). 400X magnification.