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. 2017 Jul 5;7:4695. doi: 10.1038/s41598-017-04835-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pBMP-9 and SpBMP-9 on the activation of the canonical Smad1/5 pathway. (A) Western blots of phosphorylated Smad1/5 (pSmad) and densitometric analysis of pSmad1/5 as referred to total Smad (TSmad) showing the effect of 1 nM BMP-9, pBMP-9 and SpBMP-9 +/− 10 μM RA in SH-SY5Y cells after incubation for 0, 15, 30, 60, 120 and 240 min. (B) Pictures showing the nucleus (blue) and the pSmad1/5 (red) in SH-SY5Y cells stimulated with an equimolar concentration of BMP-9, pBMP-9 or SpBMP-9 (1 nM) +/− 10 μM RA after incubation for 240 min. Results are representative of 2 independent experiments performed in duplicate (Bar = 100 μm).