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. 2017 Jul 5;7:4695. doi: 10.1038/s41598-017-04835-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pBMP-9 and SpBMP-9 on the expression and the distribution of VAchT. Merged pictures showing immunostaining for VAchT (FITC, green), actin cytoskeleton (rhodamine-phalloidin, red) and nuclei labelling (Hoechst, blue) of SH-SY5Y cells stimulated for 5d with 0, 0.1 or 1 nM BMP-9, pBMP-9 and SpBMP-9 +/− 10 μM RA. White squares show magnified zones, white arrows indicate VAchT vesicles in cell neurites (Bar = 100 μm). Pictures are representative of at least 2 independent experiments.