TABLE 2.
Health status of cattle used in this study
| Cow identifier | Disease status | Test result |
|||
|---|---|---|---|---|---|
| IDEXX ELISAa | IFN-γb | Fecal PCRc |
|||
| IS900 | ISMap02 | ||||
| 781 | Control | 0.073 | 0.063/0.077 | Neg | Neg |
| 782 | Control | 0.048 | 0.059/0.145 | Neg | Neg |
| 785 | Control | 0.058 | 0.113/0.148 | Neg | Neg |
| 53 | Subclinical | 0.046 | 0.120/0.474 | Neg | 27.95 |
| 651 | Subclinical | 0.023 | 0.124/0.279 | Neg | Neg |
| 1062 | Subclinical | 0.053 | 0.141/1.468 | Neg | Neg |
| 786 | Clinical | 2.94 | 0.044/0.084 | 26.28 | 16.62 |
| 789 | Clinical | 2.26 | 0.196/0.598 | 22.43 | Neg |
| 1442 | Clinical | 2.62 | 0.043/0.083 | 26.13 | 18.1 |
| 2222 | Clinical | 2.96 | 0.178/1.479 | 25.83 | 20.66 |
| 2444 | Clinical | 3.07 | 0.089/0.124 | 30.05 | 20.64 |
| 2491 | Clinical | 3.26 | 0.088/0.103 | 33.74 | 19.4 |
a
A negative ELISA result is defined as less than 0.1.
b
Values are reported as no stimulation/antigen stimulation. A negative IFN-γ test result is anything less than 0.11.
c
A negative (Neg) fecal PCR result is a threshold cycle (CT) value higher than 35. IS900 has 17 copies per genome, while ISMap02 has 6 copies per genome.