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. 2017 Apr 28;25(7):1588–1595. doi: 10.1016/j.ymthe.2017.04.005

Figure 2.

Figure 2

Accumulation of MicroRNA Mimics in Liver Tumor by NCP Delivery System

Biodistribution of microRNA mimics in a liver metastases model. (A–C) Ex vivo fluorescence images. TdTomato-labeled HCT116L2T colorectal cancer cells are depicted in red (excitation/emission, 535/580 nm). Alexa647-labeled microRNA mimics are depicted in green (excitation/emission, 640/680 nm). (A) Representative images of harvested whole livers at 3 hr after injection of NCPs carrying microRNA mimics demonstrate microRNA accumulation in the liver tumor area. (B) Livers, lungs, kidneys, and hearts harvested 3 hr after injection of NCPs carrying microRNA mimics. Ex vivo fluorescence imaging shows microRNA accumulation localized to liver tumors. (C) Ex vivo fluorescence images of harvested livers 3, 24, and 72 hr after injection of NCPs carrying microRNA mimics demonstrated microRNA accumulation at all time points. (D) Confocal microscopic images of HCT116L2T tumor cells showing microRNA accumulation in cytosol and preferential perinuclear microRNA localization. TdTomato fluorescence from HCT116L2T cells is depicted in red, and Alexa647 fluorescence from microRNA mimics is depicted in green. Scale bars, 20 μm (horizontal images); 51.3 μm (vertical images).