Figure 8.

Lipid Nanoparticle pH Nanosensor Measurements

(A) pH of DNA in Lipofectamine 2000 nanoparticles introduced to HEK293T cells was determined to average approximately 6.0 at all time points following transfection. (B and C) There was only a minor statistical difference between the (B) the median pH of cells that were DsRed⁺ and DsRed⁻ at 24 hr post-transfection, which was only slightly evident when looking at a (C) representative distribution of the populations. (D) Likewise to bPEI and PLL there was no highly significant correlation between DsRed expression and measured pH for the cell at 24 hr post-transfection. (E) Cell pH and DsRed gene expression showed weak (0.2 < PCC < 0.4) correlation using PCC, and no significant correlation was observed between the other relationships. Error bars show mean ± SEM of four wells.