Figure 3.

I₆P₇-Stp-His/DNA NPs Improve Gene Expression in U87 Cells and Glioma Spheroid Penetration In Vitro

(A–H) Gene expression of I₆P₇-Stp-His/pEGFP and I₆P₇scr-Stp-His/pEGFP NPs in U87 glioma cells (A–D) and 1800 astrocyte cells (E–H). (A, C, E, and G) I₆P₇-Stp-His/pEGFP NPs. (B, D, F, and H) I₆P₇scr-Stp-His/pEGFP NPs. (A), (B), (E), and (F) are corresponding bright-field images of fluorescent images (C), (D), (G), and (H). Green represents EGFP. (I–L) Glioma spheroid penetration of I₆P₇-Stp-His/DNA and I₆P₇scr-Stp-His/DNA NPs after 12 hr incubation. (I and K) I₆P₇-Stp-His/DNA NPs. (J and L) I₆P₇scr-Stp-His/DNA NPs. (I) and (J) are the corresponding 2D images of glioma spheroid. (K) and (L) are the corresponding 2.5D images of glioma spheroid obtained from the middle of z planes. DNA was pre-labeled by YOYO-3. Red represents YOYO-3. Scale bars, 200 μm.