Figure 5.

Binding, Endocytosis, and Lysosomal Trafficking of Anti-ICAM/ASM NCs In Vivo

Transmission electron microscopy of wild-type mouse lungs (A), spleen (B), and liver (C) showing anti-ICAM/ASM polystyrene NCs (pseudocolored in green) interacting with endothelial cells (ECs), splenic parenchyma cells (SP), and hepatocytes (HP), 3 hr after i.v. injection. NCs can be seen being engulfed by cells (black arrows), within cell endosomes (white arrowheads) and lysosomes (black arrowheads), and transcytosed across the endothelium into subjacent epithelial cells (white arrow). VL, vessel lumen; Cv, caveolar vesicles; Cl, clathrin vesicles; Cj, cell junction. Scale bars, 300 nm.