Fig. 3.

Influence of various methods on the percentage of LDGs in PBMCs

Dot plots of CD15 staining (X-axis) versus CD14 staining (Y-axis) show characteristics of PBMC separated by the PBMC adherence method (a, b) and the whole blood sedimentation method (c, d). (e) PBMCs were isolated by density-gradient centrifugation over Histopaque-1077 for 30 min at 400g, 600g, and 800g RCF. (f) The LDG percentages in PBMCs of the standard method and PBMC adherence method were compared. (g) The LDG percentages in PBMCs of the standard method and whole blood sedimentation method were compared. Adherence: PBMC adherence method; Sediment: whole blood sedimentation method; Standard M: standard method. Statistical analyses were performed using the paired t-test