Table 3.
Primary antibodies used in the present study including reference, supplier, host and dilution
| Antibody (anti-) | Reference | Supplier | Host | Dilution |
|---|---|---|---|---|
| β-actin | A5316 | Sigma-Aldrich, St Louis, MO, USA | Ms | 1/30000 |
| eIF2α (eukaryotic initiation factor 2α) | 5A5 | Thermo Fisher Sci, Waltham, MA, USA | Ms | 1/50 |
| eIF3η (eukaryotic initiation factor 3η) | sc 2887 | Santa Cruz, Dallas, TX, USA | Rb | 1/200 |
| eIF5 (eukaryotic initiation factor 5) | sc.282 | Santa Cruz, Dallas, TX, USA | Rb | 1/400 |
| eEF1A (eukaryotic elongation factor 1A) | 2551 | Cell Signaling, Danvers, MA, USA | Rb | 1/100 |
| eEF2 (eukaryotic elongation factor 2) | 2332 | Cell Signaling, Danvers, MA, USA | Ms | 1/1,000 |
| GAPDH (glyceraldehyde 3-phosphate dehydrogenase) | ADI-CSA-335-E | Enzo Life Sciences, Incl., Farmingdale, NY, USA | Ms | 1/5000 |
| P-eIF2α (phospho-eukaryotic initiation factor 2α pSer51) | S.674.5 | Thermo Fisher Sci, Waltham, MA, USA | Rb | 1/50 |