Table 5.
mRNA expression of genes coding for selected nucleolar and ribosomal proteins, rRNA18S and rRNA28S in the somatosensory cortex of WT and APP/PS1 mice aged 3, 12 and 20 months
| 3 months | 12 months | 20 months | ||||
|---|---|---|---|---|---|---|
|
| ||||||
| WT | APP/PS1 | WT | APP/PS1 | WT | APP/PS1 | |
| Ubtf | 1.057 ± 0.111### | 1.123 ± 0.211 | 1.628 ± 0.041 | 1.463 ± 0.083 | 1.342 ± 0.089 | 3.796 ± 0.873 |
| Npm3 | 1.188 ± 0.305 | 1.023 ± 0.087& | 0.862 ± 0.070 | 0.852 ± 0.082 | 2.518 ± 0.396 | 3.796 ± 0.873 |
| Ncl | 1.010 ± 0.059 | 1.023 ± 0.087 | 0.082 ± 0.070& | 1.003 ± 0.238 | 0.960 ± 0.086 | 1.370 ± 0.080**↑ |
| rRNA18S | 1.018 ± 0.075### | 1.085 ± 0.164& | 2.730 ± 0.188&&& | 2.237 ± 0.456 | 1.259 ± 0.170 | 2.504 ± 0.496*↑ |
| rRNA28S | 1.009 ± 0.052###,&&& | 1.076 ± 0.150 | 2.367 ± 0.191 | 1.847 ± 0326 | 1.338 ± 0.175 | 2.401 ± 0.344*↑ |
| Rpl5 | 1.004 ± 0.032### | 1.005 ± 0.038### | 3.015 ± 0.327&&& | 2.393 ± 0.122&&& | 0.727 ± 0.047 | 1.190 ± 0.147 |
| Rpl7 | 1.032 ± 0.093 | 1.029 ± 0.094 | 1.785 ± 0.279 | 1.305 ± 0.108 | 1.251 ± 0.211 | 1.366 ± 0.211 |
| Rpl21 | 1.003 ± 0.031 | 1.018 ± 0.067 | 0.903 ± 0.113 | 0.635 ± 0.058 | 0.834 ± 0.116 | 0.956 ± 0.094 |
| Rpl22 | 1.004 ± 0.037 | 1.010 ± 0.049 | 1.043 ± 0.180 | 0.758 ± 0.51 | 0.823 ± 0.073 | 1.011 ± 0.100 |
| Rpl23a | 1.009 ± 0.052 | 1.015 ± 0.049&&& | 1.081 ± 0.085 | 0.794 ± 0.036&&& | 1.005 ± 0.059 | 1.649 ± 0.108**↑ |
| Rpl26 | 1.024 ± 0.022 | 1.009 ± 0.49 | 1.459 ± 0.204 | 1.116 ± 0.108& | 1.025 ± 0.172 | 1.679 ± 0.205 |
| Rpl27 | 1.003 ± 0.28 | 1.004 ± 0.030 | 1.465 ± 0.158 | 1.255 ± 0.133 | 0.960 ± 0.434 | 1.129 ± 0.120 |
| Rpl30 | 1.023 ± 0.098# | 1.010 ± 0.049 | 0.729 ± 0.068 | 0.878 ± 0.205 | 0.838 ± 0.082 | 1.589 ± 0.263 |
| Rps3a | 1.001 ± 0.013## | 1.000 ± 0.017 | 1.683 ± 0.199&& | 1.384 ± 0.194 | 0.896 ± 0.036 | 1.336 ± 0.155*↑ |
| Rps5 | 1.002 ± 0.028## | 1.006 ± 0.043#,& | 1.987 ± 0.252& | 1.698 ± 0.231 | 1.050 ± 0.053 | 1.576 ± 0.173*↑ |
| Rps6 | 1.003 ± 0.043 | 1.191 ± 0.245 | 1.184 ± 0.080 | 1.643 ± 0.127 | 1.006 ± 0.052 | 1.831 ± 0.255*↑ |
| Rps10 | 1.007 ± 0.045 | 1.013 ± 0.065### | 3.730 ± 0.522&&& | 3.344 ± 0.368 | 1.122 ± 0.083 | 1.884 ± 0.263*↑ |
| Rps13 | 1.001 ± 0.015### | 1.004 ± 0.038# | 0.803 ± 0.092&& | 0.610 ± 0.021 | 0.778 ± 0.033 | 1.150 ± 0.128*↑ |
| Rps16 | 1.004 ± 0.032# | 1.006 ± 0.042& | 1.286 ± 0.106&& | 1.268 ± 0.120 | 0.827 ± 0.057 | 1.433 ± 0.176*↑ |
| Rps17 | 1.004 ± 0.031 | 1.008 ± 0.049 | 1.202 ± 0.096 | 1.057 ± 0.084 | 0.901 ± 0.102 | 1.225 ± 0.154 |
The non-parametric Mann-Whitney test was performed to compare each group when the samples did not follow a normal distribution whereas the unpaired t test was used for normal variables. Data are represented as the mean values ± SD.
P<0.05 compared with WT animals;
P<0.01 compared with WT animals.
P<0.05 compared with 20 months;
P<0.01 compared with 20 months;
P<0.001 compared with 20 months.
P<0.05 compared with animals aged 12 months;
P<0.01 compared with animals aged 12 months;
P<0.001 compared with animals aged 12 months.
Expression levels were calculated using Hprt for normalization. ↑ indicate gene up-regulation.