Figure 7.

Inhibitory effects of FSCP on TNF-α-induced activation of the NF-κB signaling pathway. Western blot analysis showed NF-κB p65 levels were decreased in the cytoplasm and increased in the nucleus in TNF-α-treated HaCaT cells (a). Immunofluorescence microscopic assay revealed that FSCP prevented the translocation of NF-κB p65 to the nucleus in TNF-α-treated HaCaT cells (b). Results are presented as the means ± SD of three independent experiments. CE: cytoplasmic extracts; NE: nuclear extract. ^#p < 0.05 versus the control and ^∗∗p < 0.01 and ^∗∗∗p < 0.001 versus the TNF-α-treated group.