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A The multiple sequence alignment of the p.R65Q mutant protein reveals the conservation of arginine at this position throughout evolution. B Multiple sequence alignment of the p.R119H mutant CRYAA protein reveals the conservation of R119 across species. C In silico prediction of both variants using prediction tools: SIFT, SNAP, Polyphen-1, Polyphen-2, PhD-SNP, MAPP, and PredictSNP.
