Table 2.

Oligonucleotide primers used in this study

Primer	Sequence (5′–3′)a	Restriction site
aldB-uF	AGGGTACCGGCGAAAGTCATGTAACAATCC	KpnI
aldB-uR	CTGACATGATATTTCTCTTTTCTAT
aldB-dR	CCGCTCGAGTGCTGACAGATGGCTGGCTGTG	XhoI
aldB-dF	GAAAAGAGAAATATCATGTCAGTAATTGCTTAAATTTCTTTAGC
aldB-testF	ATATTTCTGCCACAATTTTCATGCC
aldB-testR	CCAATCCTGTACCAATAACAGCAAT
pheSF	ATAAAAAATCGAAAAGGAGATAAAAATGAACTTACAAGAAAAAATTGAAG
pheSR	AAAGATCTTCAGTCGAATTGTTCTAAGAATC	BglII
pheSR2	AAGAATTCTCAGTCGAATTGTTCTAAGAATC	EcoRI
siteR	ACCAAAACCAGAATAAACAGAAG
siteF	CTGTTTATTCTGGTTTTGGTTTTGGACTCGGTCAAGAACG
ldhF1	CCGAATTCATTCATTTTACACATTGTA	EcoRI
ldhF2	GACAGATCTATTCATTTTACACATTGTA	BglII
ldhF3	AGACGTCGACATTCATTTTACACATTGTA	SalI
ldhR1	TTTTATCTCCTTTTCGATTTTTTAT
ldhR2	CGGGATCCTTTTATCTCCTTTTCGATTTTTTAT	BamHI
ldhR3	CCGCTCGAGTTTTATCTCCTTTTCGATTTTTTAT	XhoI
upF	AGGGTACCATGTCAATAGTTGTCGAAAA	KpnI
upR	CAGTTTCTGCTAAGGTATCA
downF	TGATACCTTAGCAGAAACTGATGAATTAGCACAGCAAGTG
downR	CCGCTCGAGCTCTAGTAAAATGTTCCTCA	XhoI
testF	TTAAGGAAATGAATTTAGAGGAGAG
testR	AAACCTTCATGTCCTTCTTGAGT
BL-pheSF	AAAGATCTATGGATCTTCAAACCAAACTTG	BglII
BL-siteR	ACCAAAACCGCCGTAAACGTC
BL-siteF	GACGTTTACGGCGGTTTTGGTTTTGGCCTTGGTCCTGATCG
BL-pheSR	GATCTGCAGTTAACCCTCCTGGCTGAATTGC	PstI
gfpF	ATAAAAAATCGAAAAGGAGATAAAAAGATATGAGCAAAGGAG
gfpR	CGCGAATTCTTAGTAGAGCTCATC	EcoRI

^aThe restriction sites in the primer sequences are underlined