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. 2017 Jul 6;15:58. doi: 10.1186/s12915-017-0396-0

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© Tolias et al. 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3. — Collapse of cell body during aspiration into patch pipette. Two example neurons patched in vivo under two-photon guidance using a green fluorescent dye in the patch pipette. In both cases, the cell body was noted to decrease in size dramatically after aspirating the cell contents into the pipette. Adapted by permission from Macmillan Publishers Ltd: Nature Biotechnology [1], copyright (2016)