Fig. 4.

Bead binding time point analysis. a Pre-PCR assessment. Various gDNA input amounts (X-axis) were used and libraries were made where the binding time for each of the bead cleanups was varied. The cumulative effect after all cleanups up to the point of post-ligation cleanups is shown. The purified ligated DNA was measured using a Qubit HS assay. The values from this assay were normalized to that of the 15 min condition. b Post-PCR assessment. As in (a) but purified DNA was measured after PCR enrichment. i.e. after additional two post-PCR bead-based purifications. n = 3; error bars = Standard Deviation. *P < 0.05