Fig. 9.

Evaluation of the new ssRNA-seq pipeline using RNA from tumor samples. 100 ng total RNA from 12 different tumor samples was used as input to generate libraries using the new protocol. Adapter-ligated libraries were enriched with 13 cycles of PCR. a Library yield. b Correlation of expression. Pearson’s correlation coefficient was calculated pair-wise showing higher correlation between the new lower input libraries and their previous higher input counterparts