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. 2017 Jun 5;10(6):598–604. doi: 10.14202/vetworld.2017.598-604

Table-1.

Primers used for detection of antibiotic resistant genes in S. aureus.

Gene Oligonucleotide sequence (5`-3`) Amplicon size References
aacA-aphD TAA TCC AAG AGC AAT AAG GGC
GCC ACA CTA TCA TAA CCA CTA
227 [5]
ermA AAG CGG TAA ACC CCT CTG A
TTC GCA AAT CCC TTC TCA AC
190 [5]
ermB CTATCTGATTGTTGAAGAAGGATT
GTTTACTCTTGGTTTAGGATGAAA
142 [6]
ermC AAT CGT CAA TTC CTG CAT GT
TAA TCG TGG AAT ACG GGT TTG
299 [5]
tetK GTA GCG ACA ATA GGT AAT AGT
GTA GTG ACA ATA AAC CTC CTA
360 [5]
tetM AGT GGA GCG ATT ACA GAA
CAT ATG TCC TGG CGT GTC TA
158 [5]
tetL GTCGTTGCGCGCTATATTCC
GTGAACGGTAGCCCACCTAA
696 [7]
tetO AATGAAGATTCCGACAATTT
CTCATGCGTTGTAGTATTCCA
781 [7]
mecA AAA ATC GAT GGT AAA GGT TGG C
AGT TCT GCA GTA CCG GAT TTG C
532 [5]
blaZ ACT TCA ACA CCT GCT GCT TTC
TGA CCA CTT TTA TCA GCA ACC
173 [6]
vanA ATGAATAGAATAAAAGTTGC
TCACCCCTTTAACGCTAATA
1032 [8]
16SrDNA (Staphylococcus genus specific) CAG CTC GTG TCG TGA GAT GT
AAT CAT TTG TCC CAC CTT CG
420 [5]
Coa ATA GAG ATG CTG GTA CAG G
GCT TCC GAT TGT TCG ATG C
547 (550-875) [9]
Nuc (Species specific) GCGATTGATGGTGATACGGTT
AGCCAAGCCTTGACGAACTAAAGC
279 [10]

S. aureus=Staphylococcus aureus