Table-2.
Treatments | Parameters | |||||
---|---|---|---|---|---|---|
VCO% SL% | Time of chilling (h) | Motility% | Morphology% | Viability% | Membrane integrity % | Acrosome integrity% |
C− | 24 | 11.25±2.83d | 98.54±0.45 | 10.00±2.76d | 8.50±1.90c | 61.17±1.62c |
TA | 24 | 76.08±1.80b | 97.75±0.55 | 72.42±1.86b | 69.75±1.43ab | 86.79±1.14a |
TB | 24 | 81.00±1.58ab | 98.00±0.42 | 76.00±1.70ab | 72.08±1.45a | 86.46±1.71a |
TC | 24 | 83.58±1.70a | 97.46±0.54 | 79.75±1.38a | 74.13±1.66a | 86.67±1.45a |
TD | 24 | 82.42±1.09a | 97.77±0.38 | 77.88±1.10a | 72.42±0.98a | 87.75±1.33a |
C+ | 24 | 68.92±1.70c | 98.25±0.33 | 67.04±1.61c | 65.83±1.23b | 77.92±0.98b |
C− | 72 | 10.25±2.84c | 98.33±0.36 | 7.71±2.27c | 6.67±2.29c | 63.88±2.58b |
TA | 72 | 58.67±2.58b | 98.29±0.42 | 56.50±2.32b | 54.83±2.54b | 73.25±1.82a |
TB | 72 | 63.50±3.18ab | 98.17±0.33 | 59.29±2.42ab | 58.71±2.73ab | 77.50±1.76a |
TC | 72 | 69.25±2.38a | 97.96±0.38 | 64.83±2.03a | 62.29±2.14a | 75.50±1.99a |
TD | 72 | 62.75±2.58ab | 98.29±0.33 | 58.58±1.86ab | 59.13±2.24ab | 74.46±1.49a |
C+ | 72 | 62.67±2.00ab | 98.33±0.28 | 60.46±1.41ab | 59.67±1.68ab | 72.00±1.39a |
C− | 144 | 1.42±0.67d | 98.71±0.40 | 1.79±0.62c | 1.42±0.38c | 58.08±1.25c |
TA | 144 | 31.83±1.66abc | 98.25±0.51 | 27.42±0.97ab | 27.04±1.47ab | 64.71±1.37a |
TB | 144 | 33.67±1.69ab | 98.38±0.32 | 27.59±1.39ab | 28.46±1.57ab | 63.92±1.48ab |
TC | 144 | 36.50±1.72a | 98.33±0.31 | 30.42±2.18a | 31.08±1.57a | 63.00±1.20ab |
TD | 144 | 31.00±1.91bc | 98.21±0.29 | 26.67±1.84ab | 27.00±1.96ab | 60.08±1.20bc |
C+ | 144 | 28.67±1.72c | 98.75±0.31 | 24.75±1.59b | 25.00±1.77b | 60.92±1.92abc |
p value | Treatments | 0.0001 | 0.3840 | 0.0001 | 0.0001 | 0.0001 |
Time | 0.0001 | 0.1095 | 0.0001 | 0.0001 | 0.0001 | |
Treatments*Time | 0.0001 | 0.9949 | 0.0001 | 0.0001 | 0.0001 |
Values with different superscripts within column at the same time show a significant difference at p<0.05. VCO=Virgin coconut oil, SL=Soybean lecithin, C−=0% SL with 2% VCO in Tris-based extender, TA=1% SL with 2% VCO in Tris-based extender, TB=1.25% SL with 2% VCO in Tris-based extender, TC=1.5% SL with 2% VCO in Tris-based extender, TD=1.75% SL with 2% VCO in Tris-based extender, C+=20% egg yolk in Tris-based extender, SEM=Standard error of mean