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. 2017 Jun 20;10(6):672–678. doi: 10.14202/vetworld.2017.672-678

Table-2.

Effect of different concentrations of SL and VCO in Tris-based extender on bull’s post-chilled sperm parameters after 24, 72, and 144 h (Mean±SEM).

Treatments Parameters


VCO% SL% Time of chilling (h) Motility% Morphology% Viability% Membrane integrity % Acrosome integrity%
C− 24 11.25±2.83d 98.54±0.45 10.00±2.76d 8.50±1.90c 61.17±1.62c
TA 24 76.08±1.80b 97.75±0.55 72.42±1.86b 69.75±1.43ab 86.79±1.14a
TB 24 81.00±1.58ab 98.00±0.42 76.00±1.70ab 72.08±1.45a 86.46±1.71a
TC 24 83.58±1.70a 97.46±0.54 79.75±1.38a 74.13±1.66a 86.67±1.45a
TD 24 82.42±1.09a 97.77±0.38 77.88±1.10a 72.42±0.98a 87.75±1.33a
C+ 24 68.92±1.70c 98.25±0.33 67.04±1.61c 65.83±1.23b 77.92±0.98b
C− 72 10.25±2.84c 98.33±0.36 7.71±2.27c 6.67±2.29c 63.88±2.58b
TA 72 58.67±2.58b 98.29±0.42 56.50±2.32b 54.83±2.54b 73.25±1.82a
TB 72 63.50±3.18ab 98.17±0.33 59.29±2.42ab 58.71±2.73ab 77.50±1.76a
TC 72 69.25±2.38a 97.96±0.38 64.83±2.03a 62.29±2.14a 75.50±1.99a
TD 72 62.75±2.58ab 98.29±0.33 58.58±1.86ab 59.13±2.24ab 74.46±1.49a
C+ 72 62.67±2.00ab 98.33±0.28 60.46±1.41ab 59.67±1.68ab 72.00±1.39a
C− 144 1.42±0.67d 98.71±0.40 1.79±0.62c 1.42±0.38c 58.08±1.25c
TA 144 31.83±1.66abc 98.25±0.51 27.42±0.97ab 27.04±1.47ab 64.71±1.37a
TB 144 33.67±1.69ab 98.38±0.32 27.59±1.39ab 28.46±1.57ab 63.92±1.48ab
TC 144 36.50±1.72a 98.33±0.31 30.42±2.18a 31.08±1.57a 63.00±1.20ab
TD 144 31.00±1.91bc 98.21±0.29 26.67±1.84ab 27.00±1.96ab 60.08±1.20bc
C+ 144 28.67±1.72c 98.75±0.31 24.75±1.59b 25.00±1.77b 60.92±1.92abc
p value Treatments 0.0001 0.3840 0.0001 0.0001 0.0001
Time 0.0001 0.1095 0.0001 0.0001 0.0001
Treatments*Time 0.0001 0.9949 0.0001 0.0001 0.0001
a,b,c,d

Values with different superscripts within column at the same time show a significant difference at p<0.05. VCO=Virgin coconut oil, SL=Soybean lecithin, C−=0% SL with 2% VCO in Tris-based extender, TA=1% SL with 2% VCO in Tris-based extender, TB=1.25% SL with 2% VCO in Tris-based extender, TC=1.5% SL with 2% VCO in Tris-based extender, TD=1.75% SL with 2% VCO in Tris-based extender, C+=20% egg yolk in Tris-based extender, SEM=Standard error of mean