Table-3.
Treatments | Parameters | |||||
---|---|---|---|---|---|---|
VCO% SL% | Time of freezing (days) | Motility % | Morphology % | Viability % | Membrane integrity % | Acrosome integrity % |
C− | 7 | 3.17±1.22b | 98.25±0.29 | 9.29±1.36c | 2.83±1.03b | 63.88±1.41b |
TA | 7 | 13.92±4.48b | 97.17±0.69 | 28.83±5.96b | 12.88±4.07b | 69.54±1.40b |
TB | 7 | 13.92±4.51b | 97.54±0.39 | 29.54±5.59b | 12.71±4.18b | 68.63±2.02b |
TC | 7 | 10.00±4.01b | 97.96±0.50 | 23.83±6.27b | 8.92±3.67b | 67.88±2.03b |
TD | 7 | 9.33±3.04b | 97.92±0.46 | 22.54±5.35bc | 7.88±2.58b | 63.08±5.86b |
C+ | 7 | 51.17±2.85a | 98.29±0.32 | 56.04±1.98a | 50.25±2.71a | 85.63±1.32a |
C− | 14 | 2.67±0.98c | 98.50±0.28 | 8.42±0.82c | 2.46±0.82b | 65.58±1.56b |
TA | 14 | 13.25±4.14b | 97.78±0.52 | 28.46±5.60b | 12.00±3.71b | 68.63±2.02b |
TB | 14 | 12.50±4.24bc | 98.00±0.32 | 27.71±5.09b | 11.92±3.98b | 67.83±2.35b |
TC | 14 | 9.00±3.86bc | 97.96±0.50 | 21.88±5.96b | 8.33±3.48b | 68.21±2.24b |
TD | 14 | 8.67±2.87bc | 98.08±0.39 | 19.17±4.69bc | 9.79±4.37b | 67.75±1.62b |
C+ | 14 | 50.33±2.79a | 98.46±0.26 | 55.79±2.27a | 50.17±2.63a | 84.46±1.08a |
p value | Treatments | 0.0001 | 0.2600 | 0.0001 | 0.0001 | 0.0001 |
Time | 0.6716 | 0.1617 | 0.5932 | 0.9449 | 0.6590 | |
Treatments*Time | 1.0000 | 0.9880 | 0.9995 | 0.9984 | 0.8226 |
Values with different superscripts within column at the same time show a significant difference at p<0.05. VCO=Virgin coconut oil, SL=Soybean lecithin, C−=0% SL with 2% VCO in Tris-based extender, TA=1% SL with 2% VCO in Tris-based extender, TB=1.25% SL with 2% VCO in Tris-based extender, TC=1.5% SL with 2% VCO in Tris-based extender, TD=1.75% SL with 2% VCO in Tris-based extender, C+=20% egg yolk in Tris-based extender, SEM=Standard error of mean