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. 2017 Jun 20;10(6):672–678. doi: 10.14202/vetworld.2017.672-678

Table-3.

Effect of different concentrations of SL and VCO in Tris-based extender on bull’s post thawed sperm parameters after 7 and 14 days (mean±SEM).

Treatments Parameters


VCO% SL% Time of freezing (days) Motility % Morphology % Viability % Membrane integrity % Acrosome integrity %
C− 7 3.17±1.22b 98.25±0.29 9.29±1.36c 2.83±1.03b 63.88±1.41b
TA 7 13.92±4.48b 97.17±0.69 28.83±5.96b 12.88±4.07b 69.54±1.40b
TB 7 13.92±4.51b 97.54±0.39 29.54±5.59b 12.71±4.18b 68.63±2.02b
TC 7 10.00±4.01b 97.96±0.50 23.83±6.27b 8.92±3.67b 67.88±2.03b
TD 7 9.33±3.04b 97.92±0.46 22.54±5.35bc 7.88±2.58b 63.08±5.86b
C+ 7 51.17±2.85a 98.29±0.32 56.04±1.98a 50.25±2.71a 85.63±1.32a
C− 14 2.67±0.98c 98.50±0.28 8.42±0.82c 2.46±0.82b 65.58±1.56b
TA 14 13.25±4.14b 97.78±0.52 28.46±5.60b 12.00±3.71b 68.63±2.02b
TB 14 12.50±4.24bc 98.00±0.32 27.71±5.09b 11.92±3.98b 67.83±2.35b
TC 14 9.00±3.86bc 97.96±0.50 21.88±5.96b 8.33±3.48b 68.21±2.24b
TD 14 8.67±2.87bc 98.08±0.39 19.17±4.69bc 9.79±4.37b 67.75±1.62b
C+ 14 50.33±2.79a 98.46±0.26 55.79±2.27a 50.17±2.63a 84.46±1.08a
p value Treatments 0.0001 0.2600 0.0001 0.0001 0.0001
Time 0.6716 0.1617 0.5932 0.9449 0.6590
Treatments*Time 1.0000 0.9880 0.9995 0.9984 0.8226
a,b,c

Values with different superscripts within column at the same time show a significant difference at p<0.05. VCO=Virgin coconut oil, SL=Soybean lecithin, C−=0% SL with 2% VCO in Tris-based extender, TA=1% SL with 2% VCO in Tris-based extender, TB=1.25% SL with 2% VCO in Tris-based extender, TC=1.5% SL with 2% VCO in Tris-based extender, TD=1.75% SL with 2% VCO in Tris-based extender, C+=20% egg yolk in Tris-based extender, SEM=Standard error of mean