Table 1.

Data sets used in the study. The table shows the number of peptides, subjects and alleles in each data set used in the study. The first set (column 1) encompassed response data, when reactivity of each of the 191 peptides was determined in a set of 87 HLA typed donors and was used to infer restrictions by the RATE approach. The second data set (column 2) entailed the experimental determination of HLA restriction of the same 191 peptides in 63 donors by the use of HLA transfected cell lines and was used in validation of RATE. The third data set (column 3) came from data on allergen-epitope T cell reactivity from a cohort of Timothy grass allergic donors

	ELISPOT data (for determining HLA restriction using RATE) (MTB)a	ELISPOT data (experimentally determined HLA restrictions)(MTB)b	Validation data (TG)
No. of peptides	191	191	66
No. of subjects/donors	87	63	137
No. of unique alleles	111	89	99
No. of allele-peptide combinations	21,201	16,999	6,534

^aAdditional file 1 shows the input data for RATE that was used for determining HLA restriction using RATE approach. Additional file 2 shows the complete results of this RATE analysis for all 21,201 peptide/allele combinations

^bBased on availability of HLA transfected cell lines, restrictions were experimentally determined for 3,195 HLA/peptide/donor combinations. After screening of the data as mentioned in results, 102 entries were selected for validation. This data is shown in Additional file 3