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. 2017 May 4;7(7):2047–2054. doi: 10.1534/g3.117.041095

Figure 2.

Figure 2

Confirmation of excision of the pyrG marker. The MSL27 (carRPΔ::pyrG-dpl237) strain was spot-inoculated on MMC media containing 5-FOA. After 3 d of incubation, spontaneous resistant mutants emerged forming a sector from colony (data not shown). The 5-FOA resistant mutants went through a vegetative cycle on MMC media containing 5-FOA. The obtained spores were subject to PCR analysis and auxotrophy tests. The excision of the pyrG marker from the carRPΔ::pyrG-dpl237 allele was confirmed by PCR (see text in the section of Excision of pyrG gene). The strains with the pyrG marker excised did not grow on media without uridine. Two independent carRPΔ::dpl237 strains were obtained. (1) MU402 (WT in carRP allele and pyrG−); (2) MSL27 (carRPΔ::pyrG-dpl237); (3) MSL28 (carRPΔ::dpl237); (4). MSL29 (carRPΔ::dpl237). Gene size is not to scale.