Table 1. Developmental potential of cattle embryos derived from mtDNA depleted and nondepleted Holstein donor cells, and B. taurus recipient oocytes in the presence and absence of TSA.
| Donor Cell | TSA | Fused (%) | No. Embryos | Cleaved (%)a | No. (%) Embryo Developed toa | ||
|---|---|---|---|---|---|---|---|
| 8-Cell | Morulae | Blastocysts | |||||
| Nondepleted | − | 254/271 (93.7 ± 1.6) b | 248 | 216 (87.1 ± 0.3) b,c | 152 (61.3 ± 2.2) b | 91 (36.7 ± 2.9) b | 89 (35.9 ± 3.4) b |
| + | 201/216 (93.1 ± 1.2) b | 193 | 177 (91.7 ± 3.2) b | 112 (58.0 ± 4.8) b,c | 72 (37.3 ± 1.0) b | 72 (37.3 ± 1.0) b | |
| Depleted | − | 189/247 (76.5 ± 1.7) c | 174 | 137 (78.7 ± 5.0) c | 77 (44.3 ± 4.6) c | 36 (20.7 ± 3.4) c | 35 (20.1 ± 3.8) c |
| + | 173/230 (75.2 ± 3.1) c | 163 | 144 (88.3 ± 3.6) b,c | 98 (60.1 ± 6.6) b | 58 (35.6 ± 5.0) b | 57 (35.0 ± 4.6) b | |
Values are mean ± SEM. Four replicates. All experiments were run in parallel. Different lower case letters (b and c) within a column indicate significant differences (P < 0.05, ANOVA with Duncan’s multiple range test using SAS version 9). −, absence of TSA; +, presence of TSA.
a
Percentages calculated from the number of embryos that cleaved and developed to each stage.