Experimental setup. Interspecific mating (generation P) resulting in F1 offspring occurred in the laboratory. Two pairs of F1 were crossed, forming families A and B, which were used to construct the linkage maps. Offspring from both families was collected in two consecutive breeding seasons, 2014 and 2015. Lm, Lissotriton montandoni; Lv, Lissotriton vulgaris.