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In situ hybridization analysis. (A) The GISH probe hybridized specifically to all A genome chromosomes (pink) in G. hirsutum (AtAtDtDt); 5SrDNA in red, (B–E) FISH with 45S rDNA in green. FISH analyses of somatic metaphase chromosomes from G. hirsutum (B), G. herbaceum (C), hybrid H2 (D), and hybrid H3 (E). Chromosomes were counterstained with DAPI (blue).
