Mutation calling using the high-quality W303 genome is similar to manually verified mutation numbers. Mapping was employed to compare the variant identification between the SNP-adjusted S288C draft (Lang et al. 2013) and the current high-quality genome assembly. Purple, complex (consecutive indels and polymorphisms); green, indels, red; multiple nucleotide polymorphisms (consecutive SNPs); blue, SNP. Indels, insertions/deletions; SNP, single nucleotide polymorphisms.