The validation of Vcl1 on chromosome 2. “Case” and “Control” refer to cohorts that contained the PWK/PhJ allele or an alternative allele, in the region denoted by the box (73–75 Mb). (A) The haplotype visualization of chromosome 2 for the six CC strains to generate the cases and the six CC strains to generate the controls. (B) VCL (μm/sec) at 10 min and (C) VCL at 90 min. We measured across CC founders, extinct CC lines, F0 living CC strains, F1 crosses of living CC strains, and F2 crosses of living CC strains. *** P < 0.001, ** P < 0.01, * P < 0.05. CC, Collaborative Cross; VCL, curvilinear velocity.