Table 2. Rates of interhomolog and intra/sister chromatid in cells grown in medium without added copper, and in medium with 1.4 mM copper.
Strain | Relevant genotype | Condition | CEN8-hphMX4 interhomolog | hphMX4-URA3 interhomolog | URA3-kanMX4/CAN1 interhomolog | hphMX4-kanMX4/CAN1 interhomolog | Intra/sister chromatid |
---|---|---|---|---|---|---|---|
YZ103 | Wild-type | No added copper | 1.1 × 10−5 (0.9–1.7) [1] | 5.1 × 10−6 (4.4–7.0) [1] | 6.0 × 10−6 (4.4–8.1) [1] | 1.1 × 10−5 [1] | 1.0 × 10−4 (0.7–1.2) [1] |
YZ103 | Wild-type | 1.4 mM copper sulfate | 1.3 × 10−5 (1.1–1.6) [1.2] | 8.6 × 10−5 (7.4–11) [17] | 8.8 × 10−5 (7.5–11) [15] | 1.7 × 10−4 [15] | 2.3 × 10−3 (2.0–2.7) [23] |
YZ117 | cup2/cup2 | No added copper | 8.8 × 10−6 (6.2–13) [0.8] | 3.6 × 10−6 (2.3–4.8) [0.71] | 4.2 × 10−6 (3.1–5.7) [0.7] | 7.8 × 10−6 [0.71] | 1.1 × 10−4 (0.7–1.4) [1.1] |
YZ118 | cup2/cup2 + p425-GPD-CUP1 | No added copper | 6.8 × 10−6 (4.6–10) [0.62] | 4.3 × 10−6 (2.7–4.9) [0.84] | 4.1 × 10−6 (3.1–5.2) [0.68] | 8.4 × 10−6 [0.76] | 6.7 × 10−5 (5.0–7.7) [0.67] |
YZ118 | cup2/cup2 + p425-GPD-CUP1 | 1.4 mM copper sulfate | 1.3 × 10−5 (0.9–2.5) [1.2] | 1.2 × 10−5 (1.1–1.6) [2.4] | 9.9 × 10−6 (6.9–11) [1.7] | 2.2 × 10−5 [2] | 8.4 × 10−5 (7.3–10) [0.84] |
Numbers in parentheses after the rate estimates are the 95% C.I. Numbers in square brackets in bold are the rates divided by the rate observed in YZ103 grown in medium without added copper. The number of cells per culture was determined using synthetic dextrose-complete medium containing 0.2 mM copper.