Table 1. Mutant DDR and spindle checkpoint strains.
| Genotype | Unstable Chr. (×10−5) | Allelic rec. (×10−5) | Chr. loss (×10−4) | ||||
|---|---|---|---|---|---|---|---|
| Median (Q1, Q3) | Fold stabil. | Median (Q1, Q3) | Fold stabil. | Median (Q1, Q3) | Fold stabil. | ||
| ChrVII disome mutants | ChrVII RAD+ (wild type) | 6.0 (3.5, 7.6) | 1 | 6.8 (3.5, 10) | 1 | 3.7 (1.1, 5.2) | 1 |
| erv14 | 1.7 (1.3, 2.2) | 3.5* | 1.1 (0.6, 2.2) | 6.2* | 0.8 (0.6, 3.3) | 4.6 | |
| rad9∆ | 57 (40, 71) | 1 | 6.0 (2.8, 8.2) | 1 | 22 (16, 39) | 1 | |
| rad9∆ erv14 | 7.2 (2.5, 16) | 7.9* | 2.2 (0.83, 4.2) | 2.7* | 7.2 (3.6, 15) | 3.1* | |
| rad17∆a | 560 (410, 660) | 1 | 28 (14, 34) | 1 | 39 (34, 70) | 1 | |
| rad17∆ erv14 | 140 (110, 160) | 4.0* | 5.4 (5.4, 11) | 5.2 | 24 (11, 27) | 1.6 | |
| tel1∆a | 49 (44, 54) | 1 | 72 (59, 82) | 1 | 15 (12, 24) | 1 | |
| tel1∆ erv14 | 31 (29, 36) | 1.6* | 5.6 (3.7, 7.6) | 13* | 8.3 (7.3, 12) | 1.8 | |
| rad51∆ | 280 (240, 320) | 1 | 12 (9.2, 17) | 1 | 27 (2.2, 68) | 1 | |
| rad51∆ erv14 | 110 (98, 140) | 2.6* | 2.3 (0.0, 3.0) | 5.3* | 37 (31, 49) | 0.72 | |
| mad2∆ | 16 (15, 32) | 1 | 14 (11, 18) | 1 | 54 (32, 81) | 1 | |
| mad2∆ erv14 | 13 (10, 16) | 1.3 | 2.2 (1.7, 3.1) | 6.5* | 13 (9.8, 21) | 4.2* | |
| ChrV disome mutants | ChrV RAD+ (wild type) | 13 (9.8, 16) | 1 | 21 (17, 35) | 1 | 0.52 (0, 1.5) | 1 |
| erv14 | 14 (10, 20) | 0.93 | 2.1 (1.0, 2.3) | 10* | 2.6 (1.0, 5.7) | 0.20* | |
| rad9∆ | 300 (240, 340) | 1 | 22 (13, 31) | 1 | 170 (130, 210) | 1 | |
| rad9∆ erv14 | 210 (180, 230) | 1.4* | 3.1 (2.1, 4.2) | 7.1* | 68 (53, 96) | 2.5* | |
| rad17∆a | 1800 (1600, 1900) | 1 | 210 (150, 240) | 1 | 210 (150, 300) | 1 | |
| rad17∆ erv14 | 900 (710, 1000) | 2.0* | 45 (23, 74) | 4.7* | 80 (45, 100) | 2.6* | |
| mad2∆a | 26 (18, 27) | 1 | 17 (12, 24) | 1 | 34 (33, 42) | 1 | |
| mad2∆ erv14 | 27 (17, 31) | 0.96 | 5.2 (4.2, 6.6) | 3.3* | 8.4 (5.5, 19) | 4.0* | |
Instability frequencies and genome fold stabilization of erv14− strains normalized to their ERV14+ counterparts. Cells with a light gray background indicate genome fold stabilization increase; cells with a white background indicate decreased fold stabilization (<1.0 = increased instability) or no change in stabilization (=1.0). Statistically significant appears in boldface type. Kruskal–Wallis test, * P < 0.01. Rec., recombinant; stabil., stabilization for all tables.
a
Sample sizes: rad17∆ N = 6 one isolate; tel1∆ N = 6 one isolate; mad2∆ N = 3 three isolates; ChrV rad17∆ N = 3 three isolates; ChrV mad2∆ N = 3 two isolates, rad9∆ N = 3 one isolate; rad9∆ ERV14x2 N = 3 one isolate.