Figure 3.

SIRT3 increases with MSC differentiation. (a) Western blotting demonstrating SIRT3 expression as 3 strains of passage 3 MSCs are differentiated into adipocytes and osteoblasts using appropriate induction media for 21 days. (b) Quantification of protein expression in western blots from panel (a). Expression was quantified using ImageJ and normalized to the expression of untreated MSCs for each of the 3 strains. (c) Quantitative RT-PCR demonstrating SIRT3 mRNA expression in 3 strains of MSCs after 21 days of exposure to appropriate differentiation media. Cycle threshold (C_T) values were normalized to the combination of 3 housekeeping genes (RRN18S, GAPDH, and ACTB). Bars represent means ± SEM of 2^−ΔCT values. ^∗P < 0.05 from two-sided t-tests comparing adipocytes and osteoblasts to undifferentiated MSCs.