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. 2017 Jun 5;5(6):apps.1600123. doi: 10.3732/apps.1600123

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© 2017 Burkle and Runyon. Published by the Botanical Society of America

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-NC-SA 4.0), which permits unrestricted noncommercial use and redistribution provided that the original author and source are credited and the new work is distributed under the same license as the original.

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Fig. 1. — Example workflow to investigate how the floral VOCs produced under changing environmental conditions may influence plant–pollinator interactions. (1) Across a natural environmental gradient, or through experimental manipulations of environmental conditions (not shown), measure plant traits and pollinator visitation. (2) Quantify floral VOCs by first enclosing flowers in a container and trapping emitted VOCs on an adsorbent trap using a portable volatile collection system. (3) Then elute, identify, and measure the VOCs using GC-MS. (4) Analyze and visualize patterns in VOCs.