a–c, Tumors (a), tumor weights (b), and tumor immunoblots (c) at necropsy from mice after orthotopic injection of UMRC-2 cells that had undergone CRISPR-based editing with sgControl or sgHIF2α-6 as in Fig. 5g; sgCon, n=5 and sgHIF2α, n=5 mice from two independent experiments. The reason for the variable HIF2α levels in (c) is unknown but could reflect, at least partly, variable amounts of host-derived cells in the tumor samples. d, Levels of the indicated mRNAs, normalized to beta actin, in tumors from a–c. Data as median with range (b, d). Statistical significance was assessed by using two-tailed Student’s t-tests (b) or Mann-Whitney test (d). Loss of HIF2α did suppress subcutaneous tumor growth (data not shown).