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. Author manuscript; available in PMC: 2017 Jul 6.
Published in final edited form as: Nature. 2016 Sep 5;539(7627):107–111. doi: 10.1038/nature19795

Figure 4.

Figure 4

PT2399 antitumor activity. a, Representative BLI of orthotopic tumors formed by 786-O CMV-Luc cells before and after PT2399 30 mg/kg or vehicle twice daily by oral gavage for 30 days (vehicle, n=15 mice from three independent experiments and PT2399, n=13 mice from three independent experiments) b, Spider plot showing growth of tumors as in (a) as determined by serial BLI. For each tumor the BLI values were normalized to the corresponding day 0 value. c, Quantification of BLI from mice as in (a). For c, the value for each tumor was normalized to the pretreatment value for that tumor. d, Representative BLI of lung colonies formed by M2A-Luc cells and treated with PT2399 30 mg/kg or vehicle twice daily by oral gavage (vehicle, n=9; PT2399, n=9 from two independent experiments). e, f, Quantification of BLI values (e) and Kaplan-Meier survival curves (f) from mice treated as in (d) (vehicle, n=9 mice from two independent experiments and PT2399, n=9 mice from two independent experiments). g, Immunoblots of M2A cells infected with a lentivirus encoding HIF2α S304M or empty vector. h, Representative BLI of lung colonies formed by M2A cells in (g) treated with PT2399 30 mg/kg or vehicle twice daily by oral gavage (for EV, vehicle and PT2399, n=7 and n=8, respectively; for S304M, vehicle and PT2399, n=9 and 10, respectively). i, Quantification of BLI values as in (h); for EV, vehicle and PT2399, n=7 and n=8, respectively; for S304M, vehicle and PT2399, n=9 and 10, respectively from two independent experiments. Data as median with range(c, e and i) Statistical significance was assessed using Mann-Whitney test (c and i ), unpaired t-test (e) or log-rank test (f).